QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn

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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (31) : 295-299. DOI: 10.11924/j.issn.1000-6850.2014-1310

QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn

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Abstract

A simple, fast, and environmental-friendly determination method of alachlor residue in corn was established using QuEChERS- HPLC equipped with UV detector. Samples were extracted by a little acetonitrile, purified by N- propyl ethylenediamine (PSA) and graphitized carbon black (GCB). The concentration of alachlor was detected by HPLC-UV. The analytical column was C18 (250 mm×4.6 mm×5.0 μm) and the mobile phase was acetonitrile mixed with water (70:30 V/V). With a significant linear relation (R2≥0.994) of standard samples measured by this method, the average recovery rates of different alachlor doses (0.2-10 mg/kg) were 96.7%-113.8% and RSD were 3.2%-15.3%. The limit of detection and the limit of quantitation were 0.015 mg/kg and 0.05 mg/kg respectively. The accuracy, sensitivity and precision of this method can meet the requirement of pesticide residue analysis.

Key words

QuEChERS; alachlor; corn; HPLC

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QuEChERS-HPLC Residue Analysis Method of Alachlor in Corn. Chinese Agricultural Science Bulletin. 2014, 30(31): 295-299 https://doi.org/10.11924/j.issn.1000-6850.2014-1310

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