Response of tobacco δ13C value and carbon-nitrogen metabolism to increased UV-B radiation

Jinhan Yang,Zongyu Chen, and

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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (31) : 64-70. DOI: 10.11924/j.issn.1000-6850.2014-0682

Response of tobacco δ13C value and carbon-nitrogen metabolism to increased UV-B radiation

  • Jinhan Yang,Zongyu Chen, and
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Abstract

The paper aims to study the effect of enhanced UV- B radiation on δ13C value, carbon- nitrogen metabolism and chemical composition of tobacco leaf. Flue-cured tobacco variety‘K326’was taken as test material for enhanced UV-B radiation in field experiment in Zhaowei experimental base of Hongta District, Yuxi Prefecture, Yunnan Province. The results showed that 13 leaf position had a range of δ13C values of -26.77‰~-25.16‰under different treatments and 16 leaf position had a range of δ13C values of -26.00‰~ -24.99‰. In the natural environment, the measured value of UV-B radiation and the changed trend value of δ13C results indicated that the tobacco δ13C values decreased with UV-B radiation enhancing. Except the total carbon content of T1 reduced obviously, the rest of the total carbon content changed little. Total nitrogen content increased with ultraviolet radiation, especially obvious in T2. The UV-B radiation and total nitrogen content were significantly positive correlated (P<0.05), and the UV-B radiation and carbon-nitrogen ratio had significant negative correlation (P<0.05). The chemical compositions of the two leaf positions were scored as T2>T1>T3>CK, and T2 had the best chemical composition coordination in the overall evaluation. Correlation analysis result showed that UV-B radiation was positively correlated with nicotine, total nitrogen and potassium, while negatively correlated with the total sugar, reduced sugar and chlorine, and had significantly negative correlation with reduced sugar (P<0.05).

Key words

tobacco; UV-B radiation; δ13C; carbon-nitrogen metabolism; chemical composition

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Jinhan Yang,Zongyu Chen, and. Response of tobacco δ13C value and carbon-nitrogen metabolism to increased UV-B radiation. Chinese Agricultural Science Bulletin. 2014, 30(31): 64-70 https://doi.org/10.11924/j.issn.1000-6850.2014-0682

References

[1] Jansen M A K, Gaba V, Greenberg B M. Higher plants and UV-B radiation: Balancing damage, repair and acclimation[J]. Trends Plant Science,1998,3:131-135.
[2] Madronich S, Mekenzie R L, Bj(o)rn L O, et al. Changes in biologically active ultraviolet radiation reaching the Earth’ s surface [J]. Journal of Photochemistry and Photobiology B: Biology,1998, 46:1-27.
[3] 何承刚,杨志新,烧建平,等.增强 UV-B辐射对 2个烤烟品种主要化学成分的影响[J].中国农业生态学报,2012,20(6):767-771.
[4] Ormrod D P, Schmidt A M, Livingston N J. Effect of UV- B radiation on the shoot dry matter production of stable carbon isotope composition of two Arabidopsis thaliana genotypes[J]. Physiol Plant,1997,101:497-502.
[5] 林光辉.稳定同位素生态学:先进技术推动的生态学新分支[J].植物生态学报,2010,34(2):119-122.
[6] 郭东锋,祖朝龙,李田,等.烤烟烟叶稳定同位素与化学成分关系研究[J].中国烟草科学,2012,33(4):42-44.
[7] 单德鑫,杨书海,李淑芹,等.15N 示踪研究烤烟对氮的吸收及分配[J].中国土壤与肥料,2007(2):15-19.
[8] 易现峰,张晓爱.稳定性同位素技术在生态学上的应用[J].生态学杂志,2005,24(3):306-314.
[9] 郭培国,陈建军,郑燕玲,等.应用 15N 示踪法研究烤烟的氮素营养[J].中国烟草学报,1998(2):29-31.
[10] Kim H Y, Kobayashi K, Nouchi I, Yoneyama T. Enhanced UV- B radiation has little effect on growth, δ13C values and pigments of potgrown rice (Oryza sativa) in the field[J]. Physiology Plant,1996,96: 1-5.
[11] 颜侃,宋鹏飞,陈宗瑜,等.低纬高原两个亚生态区烤烟种植生态适应性[J].生态学杂志,2012,31(4):870-876.
[12] 谭淑文,王毅,王崇德,等.烤烟叶片稳定碳同位素组成的品种分异与超微结构的关系[J].中国农学通报,2013,29(31):83-90.
[13] 吴潇潇,王娟,王毅,等.不同亚生态烟区烤烟对δ13C值变化的响应[J].中国农学通报,2014,30(4):72-79.
[14] 颜侃,陈宗瑜,王娟,等.不同生态区烤烟叶片稳定碳同位素组成特征 [EB/OL].(2014- 06- 12) [2014- 06- 25]. http://www.cnki.net/kcms/ doi/10.5846/stxb201307311996.html.
[15] 朱宗文,查丁石,李贤,等.物理辐射在农作物育种和生理上应用的研究进展[J].江西农业学报,2010,22(6):23-26.
[16] 欧阳磊,周翼衡,陈习羽,等.UV-B辐射对烟草形态、光合色素和抗氧化酶活性的影响[J].湖南农业科学,2012(5):21-23.
[17] 钟楚,陈宗瑜,王毅,等.UV-B辐射对植物影响的分子水平研究进展[J].生态学杂志,2009(1):129-137.
[18] Bornman J F. Target sites of UV- B radiation in photosynthesis of higher plants[J]. Ournal Photochemistry and Photobiology B: Biology, 1989,4:145-158.
[19] 冯虎元,安黎哲,陈拓,等.大豆作物响应增强 UV-B辐射的品种差异[J].西北植物学报,2002,22(4):845-850.
[20] 尚志强.中国烟草育种进展及发展对策[J].内蒙古农业科技,2010 (5):1-3.
[21] 何春霞,李吉跃,张燕香,等.5种绿化树种叶片比叶重、光合色素含量和δ13C 的开度与方位差异[J].植物生态学报,2010,34(2):134- 143.
[22] 冯虎元,安黎哲,王勋陵.环境条件对植物稳定碳同位素组成的影响[J].植物学通报,2000,17(14):312-318.
[23] Farquhar G D, O’Leary M H, Berry J A. On the relationship between carbon is tope discrimination and the intercellular carbon dioxide concentration in leaves[J]. Aust J Plant Physiology,1982,9: 121-137.
[24] Bender M M. Variation in the 13C/12C ratios of plants in relation to the pathway of photosynthetic carbon dioxide fixation[J]. Photochemistry,1971,10:1239-1244.
[25] Correia C M, Pereira J M M, Coutinh O J F, et al. Ultraviolet- B radiation and nitrogen affect the photosynthesis of maize: a Mediterranean field study[J]. European Journal of Agronomy,2005, 22(3):337-347.
[26] 孙谷畴,赵平,曾小平,等.UV-B辐射对香蕉光合作用和不同氮源利用的影响[J].植物生态学报.2001,25(3)317-324.
[27] 陈育平,王军,王勋陵,等.增强 UV-B辐射与 CO2浓度倍增复合作用对冬季大棚黄瓜生长发育的影响[J].西北植物学报,2005,25(3): 525-530.
[28] 王勋陵.增强紫外 B辐射对植物及生态系统影响研究的发展趋势[J].西北植物学报,2002,22(3):670-681.
[29] Dohler G, Hagmeier E, David C H. Effect of solar and artificial UV irradiation on pigments and assimilation of 15N ammonium 15N nitrate by macro algae[J]. J Photochem Photobiol B: Biol,1995,30: 179-187.
[30] Ku Mara, Tyagi M B, Jh A P, et al. Inactivation of cyanoacterial nitrogenous after exposure to ultraviolet- B radiation[J]. Curr Microbiol,2003,46:380-384.
[31] Kilronomos J N, Allen M F. U V- B mediated changes on below ground communities associated with the root s of a cersaccharum [J]. Functional Ecol,1996,9:923-930.
[32] Kakani V G, Reddy K R, Zhao D, et al. Effect of ultraviolet let UVB radiation on cotton (Gossypium hirsutum L.) morphology and anatomy[J]. Annals of Botany,2003,91:817-826.
[33] 范晶,赵惠勋,李敏.比叶重及其与光合能力的关系[J].东北林业大学学报,2003,31(5):37-39.
[34] Dong X J, Zhang X S. Some observations of the adaptations of sandy shrubs to the arid environment in the Wu Us sandland: leaf water relations and anatomic features[J]. Journal of Arid Environments,2001,48:41-48.
[35] 刘海燕,李吉跃.稳定碳同位素在植物水分利用效率研究中的应用[J].西北林学院学报,2008,23(1):54-58.
[36] 严昌荣,韩兴国,陈灵芝.六种木本植物水分利用效率和其小生境关系研究[J].生态学报,2001,21(11):1952-1956.
[37] 王瑞新.烟草化学[M].北京:中国农业出版社,2003:114,165.
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