Cloning and Bioinformatics Analysis of the VP Gene of Porcine Kobuvirus

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Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (32) : 10-14. DOI: 10.11924/j.issn.1000-6850.2014-0555

Cloning and Bioinformatics Analysis of the VP Gene of Porcine Kobuvirus

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Abstract

To enrich the gene for the newly identified porcine kubovirus, theVP gene of porcine kobuvirus was amplified by RT-PCR method from tissues of piglets associated with diarrhea in Fujian Province and then sequenced, then bioinformatics analysis were conducted to investigate the structure and function of the porcine kubovirus VP gene. The results demonstrated that the porcine kobuvirus VP gene was 2529 bp in length, coding an open reading frame (ORF) with 843 amino acids. The isoelectric point, molecular weight and instability index of porcine kubovirusVP protein were 5.34, 89.603kD, 37.71, respectively. The maximum and the minimum hydrophobicity were 2.522 and - 2.622, respectively. Compared with the PKV VP genes published previously download from GenBank, the sequenced gene shared the highest homogeneity with XX strain, with 86.7%; while had the lowest homogeneity with swine/S-1-HUN/2007/Hungary strain, which was 83.7% . The porcine kubovirus VP gene shares two different phylogenetic relationship branches, and the Chinese porcine kubovirus isolates distribute at both branches.

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porcine kobuvirus; VP protein; bioinformatics analysis

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Cloning and Bioinformatics Analysis of the VP Gene of Porcine Kobuvirus. Chinese Agricultural Science Bulletin. 2014, 30(32): 10-14 https://doi.org/10.11924/j.issn.1000-6850.2014-0555

References

[1] Reuter G, Boros A, Pankovics P. Kobuviruses - a comprehensive review [J]. Rev Med Virol, 2011,21(1):32-41.
[2] 杨德全,葛菲菲,鞠厚斌,等.嵴病毒(Kobuvirus)研究进展[J].中国预防兽医学报,2012,34(12):1006-1009.
[3] 姜增鹤.猪嵴病毒的研究进展[J].中国猪业,2013,9:36-38.
[4] http://www.ictvonline.org/taxonomyHistory.asp?taxnode_id=20125 928&taxa_name=Aichivirus%20A
[5] http://www.ictvonline.org/taxonomyHistory.asp?taxnode_id=20125 929&taxa_name=Aichivirus%20B
[6] http://www.ictvonline.org/taxonomyHistory.asp?taxnode_id=20125 933&taxa_name=Aichivirus%20C
[7] Reuter G, Boldizsár A, Kiss I, et al. Candidate new species of Kobuvirus in porcine hosts [J]. Emerg Infect Dis, 2008, 14(12): 1968-1970.
[8] Yu J, Jin M, Zhang Q, et al. Candidate porcine Kobuvirus, China [J]. Emerg Infect Dis, 2009,15(5):823-825.
[9] 修金生,陈小权,王斌,等.猪嵴病毒实时荧光定量 RT-PCR 方法的建立[J].中国人兽共患病学报,2012,28(9):922-926.
[10] Reuter G, Kecskemeti S, Pankovics P. Evolution of porcine kobuvirus infection, Hungary [J]. Emerg Infect Dis, 2010, 16(4): 696-698.
[11] Yu JM, Xu ZQ, Li BW, et al. Analysis and characterization of the complete genome of a member of a new species of kobuvirus associated with swine [J]. Arch Virol, 2011, 156(5):747-751.
[12] Wang C, Lan D, Cui L, et al. Molecular characterization of a porcine kobuvirus strain in China [J]. Arch Virol, 2012, 157(3):573- 578.
[13] Lin Y, Xiao S, Zeng Y, et al. Complete genome sequence of porcine kobuvirus strain WUH1 [J]. J Virol, 2012, 86(12):7010.
[14] Reuter,G., Nemes,C., Boros,A.et al. Porcine kobuvirus in wild boars (Sus scrofa) [J]. Arch Virol, 2013, 158 (1): 281-282.
[15] Cao W, Zheng H, Zhang K, et al. Complete Genome Sequence of the Porcine Kobuvirus Variant CH/HNXX-4/2012 [J]. J Virol, 2012, 86 (21):11947.
[16] Fan S, Sun H, Ying Y, et al. Identification and characterization of porcine kobuvirus variant isolated from suckling piglet in gansu province, China [J]. Viruses, 2013, 5 (10): 2548-2560.
[17] Park SJ, Kim HK, Moon HJ,et al. Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea [J]. Arch Virol. 2010,155(11):1803-1811.
[18] 沈素芳,汤赛冬,孙泉云.猪 Kobu病毒感染概况[J].动物医学进展, 2012,33(1):115-116.
[19] Wang C, Lan D, Hua X. Porcine kobuvirus from pig stool specimens in Shanghai, China [J]. Virus Genes, 2011,43(3):350-352.
[20] An D J, Jeoung H Y, Jeong W, et al. Porcine kobuvirus from pig stool in Korea [J]. Virus Genes, 2011,42(2):208-211.
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