Screening and Identification of Antagonisms Against the Soilborne Pathogens

PDF(690 KB)
PDF(690 KB)
Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (29) : 8-14. DOI: 10.11924/j.issn.1000-6850.2014-0392

Screening and Identification of Antagonisms Against the Soilborne Pathogens

Author information +
History +

Abstract

To make the laying hen manure have the multi-function of preventing soil-borne diseases. We isolated 170 strains from the experimental field and laying hens farms of Anhui Academy of Agricultural. 7 strains were screened out by the plate confrontation experiment carried out to test the inhibition effect of 170 strains to Colletotrichum capsici. After that, we carried out inhibitory spectrum experiments, temperature experiments and proliferation assays with these 7 strains to screen out the effective strains. At last, better ones were taken to molecular biological identification test. The results showed that the inhibitory rates of TCC53 to Sclerotinia sclerotiorum and Rhizoctonia cerealis were 90.4% and 82.3% respectively, the inhibitory rate of TCC157 to Colletotrichum capsici and Phytophthora capsici were 88.6% and 71.2%. And both of them could be grown in more than 40℃. In the molecular biology assay, TCC53 and TCC157 were identificated as Aspergillus aculeatus and Penicillium crustosum. Which were existed in the environment commonly, could grow fast and non-pathogenic, add the 2 strains to the organic fertilizer made by laying hen manure could help the crops resistant disease and improve soil structure.

Key words

Laying Hen Manure; Biocontrol Organic Fertilizer; Aspergillus aculeatus; Penicillium Crustosum

Cite this article

Download Citations
Screening and Identification of Antagonisms Against the Soilborne Pathogens. Chinese Agricultural Science Bulletin. 2014, 30(29): 8-14 https://doi.org/10.11924/j.issn.1000-6850.2014-0392

References

[1] Hoitink H A J, Peter C F. Basis for the control of soilborne plant pathogens with composts[J].Annual Reviews of Phytopathology, 1986,24:93-114.
[2] 袁英英,李敏清,胡伟,等.生物有机肥对番茄青枯病的防效及对土壤微生物的影响[J].农业环境科学学报,2011,30(7):1344-1350.
[3] Ding C Y, Shen Q R, Zhang R F, et al. Evaluation of rhizosphere bacteria and derived bio- organic fertilizers as potential biocontrol agents against bacterial wilt (Ralstonia solanacearum) of potato[J]. Plant Soil,2013,366:453-466.
[4] 蔡燕飞,廖宗文,章家恩,等.生态有机肥对番茄青枯病及土壤微生物多样性的影响[J].应用生态学报,2003,14(3):340-353.
[5] Garbeva P, van Veen J A, van Elsas J D. Microbial diversity in soil Selection of microbial populations by plant and soil type and implications for disease suppressiveness[J].Annual Review of Phytopathology,2004,42:243-270.
[6] 程凯,江欢欢,沈标.棉花黄萎病拮抗菌的筛选及其生物防治效果[J].植物营养与肥料学报,2011,17(1):166-174.
[7] 刘会清,张爱香,马海莲.生防菌剂与生物有机肥复配对黄瓜抗病促生效果的研究[J].北方园艺,2011(5):1-4.
[8] Yang H T, Maarten R, Tang W H. Toxicity of fungicides and selective medium development for isolation and enumeration of Trichoderma spp.in agricultural soils[J]. Shandong Science,2005, 18(3):113-123.
[9] 孙虎,薛保国,杨丽荣,等.小麦全蚀病拮抗木霉 ZBS6的分离、筛选及鉴定[J].河南农业科学,2010,10:79-83,86.
[10] 于雪云.土壤中多功能木霉菌的筛选鉴定及木霉多样性分析[D].济南:山东理工大学,2007.
[11] 张慧,杨兴明,冉炜.土传棉花黄萎病拮抗菌的筛选及其生物效应[J].土壤学报,2008,45(6):1095-1101.
[12] 魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[13] 胡小加,张明海,余常兵,等.抑制油菜菌核萌发的真菌分离及其生防研究初报[J].中国油料作物学报,2010,32(4):567-570.
[14] 袁佐清.土壤中木霉的分离纯化以及对植物病原的拮抗研究[J].西北农业学报,2006,15(5):132-135.
[15] 贾振华,石万龙,田连生.从土壤中分离木霉的培养基的研究[J].河北省科学院学报,2000,17(4):232-234.
[16] 刘伟,刘鹏,沈小英,等.烟草青枯病拮抗芽孢杆菌的筛选、鉴定及其抑菌活性初探[J].西北农林科技大学学报,2014,42(2):123-130.
[17] 韦巧婕,郑新艳,邓开英,等.黄瓜枯萎病拮抗菌的筛选鉴定及其生物防效[J].南京农业大学学报,2013,36(1):40-46.
[18] 曹明慧,冉炜,杨兴明,等.烟草黑胫病拮抗茵的筛选及其生物效应[J].土壤学报,2011,48(1):151-159.
[19] 江欢欢,程凯,杨兴明,等.辣椒青枯病拮抗菌的筛选及其生物防治效应[J].土壤学报,2010,47(6):1225-1231.
[20] Zhao Q Y, Dong C X, Yang X M, et al. Biocontrol of Fusarium wilt disease for Cucumis melo melon using bio- organic fertilizer[J]. Applied Soil Ecology,2011,47(1):67-75.
[21] Ling N, Xue C, Huang Q W, et a1. Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt[J].Biological Control,2010, 55(5):673- 683.
[22] 李娟,李红丽,王岩.大白菜软腐病颉颃菌筛选及其发酵条件研究[J].湖北农业科学,2011,50(2):281-284.
Share on Mendeley
PDF(690 KB)

20

Accesses

0

Citation

Detail

Sections
Recommended

/