Safety of Morus alba and Bombyx mori to seven commonly used fungicides, 80% mancozeb WP, 70% propineb WP, 80% tebuconazole WP, 30% difenoconazole and propiconazole EC, 50% chlorothalonil WP, 50% azoxystrobin WG and 22.5% picoxystrobin SC, was evaluated, aimed to understand their safety to M. alba and B. mori. The result indicated that mulberry was safe after sprayed seven fungicides within 4 times the recommended dose range. There were intoxication of 4 times the dose of 30% difenoconazole and propiconazole EC, 50% azoxystrobin WG and 22.5% picoxystrobin SC to B. mori at after 3 d, and other treatment had not significant toxicity. Silkworm fed mulberry leaves that treated 80% mancozeb WP, 70% propineb WP and 22.5% picoxystrobin SC at 4 times the recommended dose after 3 d, which the sleepless rates were 23.44 %, 28.33% and 53.70%, respectively, and 4 and 5 instar than controls to extend 0.5-1.5 d, the cocoon weight and cocoon shell weight were significantly lower than the control, but other fungicides were not significant difference. Therefore, silkworms fed mulberry leaves sprayed by seven fungicides at least after 7 d, especially 80% mancozeb WP, 70% propineb WP and 22.5% picoxystrobin SC should control the dosage and interval.