Effects on M1 generation of rice induced by space-flight was quite different from that induced by other commonly used physical and chemical mutagens, and even the effects of M1 populations carried in different space-crafts were not exactly the same. To investigate the effects on M1 generation of rice cultivar‘Hanghui 173’(Oryza sativa L. ssp. indica) whose seeds were carried in "ShenzhouⅧ" spacecraft (SP1), seven characters including the germination rate, seedling height, plant height, length and width of flag-leaf, length and setting percentage of main-panicle were determined, and comparison of the SP1 to 250 Gy 60Co-γrays irradiated- M1 generation (G1) and 1% EMS (Ethyl Methane Sulphonate) treatment - M1 generation (E1) was conducted. Further, to study the molecular mutagenic effect of "ShenzhouⅧ" carrying, 24 SSR markers were used to screening genetic variations within 100 mixed samples from 300 SP1 individuals. The results showed that, contrast to the control, germination rate and main-panicle's setting percentage of the SP1 decreased at significant and highly significant respectively, while remained five characters were found no significant difference. Comparative analysis showed that the "ShenzhouⅧ" carrying did not have effects on the late growth of SP1 plants, whereas the late growth of G1 and E1 plants were promoted leading to significant or highly significant differences on their plant height and length and width of flag-leaf. Amplification of 24 SSR markers showed that only one variation band was detected at locus RM267, and the corresponding variation rate was 0.014%.
Key words
rice;“Shenzhou Ⅷ” ; 60Co-γ rays; EMS; SSR
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References
[1] 魏力军,王俊敏,杨谦,等.水稻空间搭载与地面γ辐照诱变效应的比较研究[J].中国农业科学,2006,39(7):1306-1312.
[2] 郑家团,谢华安,王乌齐,等.水稻航天诱变育种研究进展与应用前景[J].分子植物育种,2003,15(3):367-371.
[3] 刘录祥,郭会君,赵林姝,等.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592.
[4] 王乃彦.开展航天育种的科学研究工作,为我国农业科学技术的发展做贡献[J].核农学报,2002,16(5):257-260.
[5] 刘录祥,王晶,赵林姝,等.作物空间诱变效应及其地面模拟研究进展[J].核农学报,2004,18(4):247-251.
[6] Bayonove J, Burg M, Delpoux M, et al. Biological changes observed on rice and biological and genetic changes observed on tobacco after space flight in the orital station Salyut-7 (Biobloc III experiment)[J]. Adv Space Res,1984,4(10):97-101.
[7] Gu R Q, Shen H M. Effects of space flight on the growth and some cytological characteristics of wheat seedlings[J]. Acta Phytophysiologica Sinica,1989,15(4):403-407.
[8] Mei M, Qiu Y, Sun Y, et al. Morphological and molecular changes of maize plants after seeds been flown on recoverable satellite[J]. Adv Space Res,1998(22):1691-1697.
[9] Badhwar G. The radiation environment in low- earth orbit [J]. Radiat Res,1997(148):3-10.
[10] 黄荣庆,蒋兴村,李金国,等.在生物堆试验中用塑料核径迹探测器作高游离宇宙线重核的辐射生物学研究[J].核技术,1991,14(7): 428-429.
[11] 骆艺,王旭杰,梅曼彤,等.空间搭载水稻种子后代基因组多态性及其与空间重离子辐射关系的探讨[J].生物物理学报,2006,22(2): 131-138.
[12] Ou X, Long L, Wu Y, et al. Spaceflight- induced genetic and epigenetic changes in the rice (Oryza sativa L.) genome are independent of each other[J]. Genome,2010,53(7):524-532.
[13] 周峰,易继财,张群宇,等.水稻空间诱变后代的微卫星多态性分析[J].华南农业大学学报,2001,22(4):55-57.
[14] 蒲志刚,张志勇,郑家奎,等.水稻空间诱变的遗传变异及突变体的AFLP分子标记[J].核农学报,2006,20(6):486-489.
[15] 王丰,李永辉,柳武革,等.水稻不育系培矮 64S的空间诱变效应及后代的 SSR分析[J].核农学报,2006,20(6):449-453.
[16] Allen G, Flores- Vergara M, Krasynanski S, et al. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide[J]. Nat Protoc,2006,1(5):2320- 2325.
[17] 郭涛,霍兴,饶得花,等.水稻半矮秆基因 iga-1 的鉴定及精细定位[J].作物学报,2011,37(6):955-964.
[18] 徐建龙.空间诱变因素对不同粳稻基因型的生物学效应研究[J].核农学报,2000,14(1):56-60.
[19] 严文潮,徐建龙,俞法明,等.不同早籼基因型水稻的空间诱变效应研究[J].核农学报,2004,18(3):174-178.
[20] 王俊敏,徐建龙,魏力军,等.空间环境和地面γ辐照对水稻诱变的差异[J].作物学报,2006,32(7):1006-1010.
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Footnotes
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