Construction of Plant Bivalent Expression Vectors Against Aflatoxin in Peanut

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (6) : 124-128. DOI: 10.11924/j.issn.1000-6850.2013-1485
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Construction of Plant Bivalent Expression Vectors Against Aflatoxin in Peanut

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Abstract

The performance of species resistance to Aspergillus by conventional breeding are unstable, therefore, genetic engineering is used to breed varieties resistant to Aspergillus. CHI and GLU are broad-spectrum resistance genes and have synergistic effect. Based on constructed vectors pSC1300-8-CHI and pSC1300-13-GLU, NcoⅠrestriction site was added to the front of the promotor 8# of CHI gene and AflⅡrestriction site was added to the end of T-nos sequence through PCR amplification, ApaⅠrestriction site was added to the front of the promotor 8# of GLU gene and SpeⅠrestriction site was added to the end of T-nos sequence through PCR amplification, two genes will be connected to antibiotics self-removing carrier pLoxp after digestion, antibiotic induced-removing plant bivalent expression vectors pLoxp-8-CHI-13-GLU was constructed. Identified by restriction endonuclease, the expression vector was successfully constructed.

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tissue-specific promotors; resistance to Aspergillus; expressing vectors; antibiotic induced-removing

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Construction of Plant Bivalent Expression Vectors Against Aflatoxin in Peanut. Chinese Agricultural Science Bulletin. 2014, 30(6): 124-128 https://doi.org/10.11924/j.issn.1000-6850.2013-1485

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