Abstract: Choose four different types of Eurotium cristatum(standard Eurotium cristatum（G1）,Eurotium cristatum drape（G2）,Eurotium cristatum variecolor（G3）, Eurotium cristatum light yellow（G4）) as inoculant, vaccination to anhua dark tea we get their brick loose tea. Through the comparison of four different types of Eurotium cristatum in the inoculation to anhua dark tea 3, 7, 11 days later in EGCG,caffeine, theanine, tea polyphenols, soluble sugar, water extract and fluorine,to find the best type of Eurotium cristatum. The results show that,after vaccination to anhua dark tea 3, 7, 11 days later, the treatment of Eurotium cristatum drape’s（G2）brick loose tea is better than others. Among them, the EGCG, theanine, tea polyphenol content are higher than the other three processing, And the content of EGCG G2 and G3, G4 have significant different, caffeine content is minimum,soluble sugar content is slightly lower than other three processing. Comprehensive to see, the treatment of Eurotium cristatum drape’s（G2）brick loose tea is best,and suitable for promotion.