Pot experiment was carried out to test the differences of Cd chemical forms among different tobacco Cultivars. The Cd forms in plants shows that the FHAC proportion is highest in root, FHAC proportion decreased and FNaCl increased in stem,while tha FNaCl proportion becomes highest in leaf, which shoed the different mechanisms of cadmium tolerance in the roots, stems and leaves of tobacco. With the increase of Cd in soil, the content of every form of Cd also increased. The FHAC proportion in root of high accumulator is higher than thatof low accumulators and the FW, FNacl proportion is lower than thatof low accumulators. The FNacl proportion in leaf of tolerance accumulator is higher and Fw of tolerante accumulator is lower than that of sensitive accumulators. It implies that the complex of cadmium and protein might be the reason why some tobacco cultivars are tolerant to cadmium. the complex of cadmium and protein is transported from cytoplasm to vacuole, which is compartmented in the vacuole.