In order to investigate resources diversity of Perilla frutescens (L.) in Guizhou Province, and study on the difference and cause of leaf color trait of Perilla frutescens (L.). The 53 copes of regional cultivated and weedy type resources of Perilla frutescens (L.) in Guizhou Province were collected and cultivated, then leaf color in seedling stage of them were surveyed, and anthocyanin and chlorophyll content intheir leaves were determined. The results showed that: there were various characters of leaf color in Guizhou regional Perilla resources, such as front green and back purple, total green and total red (purple) and so on. The higher content of anthocyanin and lower content of chlorophyll were determined in total red (purple) Perilla, and opposite results were determined in total green Perilla. The maximize differences of anthocyanin content between various materials could reach 60 times, the chlorophyll content differences only reach 3 times. However, there were two materials hold higher content of both anthocyanin and chlorophyll. The different anthocyanin and chlorophyll content led to that the larger diversity of leaf color characters in Guizhou regional Perilla resources. This work could lay a foundation of the research on resource diversity in Perilla frutescens (L.)