The current experiment was conducted to screen out novel jute lines with high seed yield per plant at seed mature stage and high fiber yield at fiber mature stage. 14 new jute lines introduced from Fujian Agriculture and Forestry University were used in the comparative experiment to investigate agronomic traits, economic traits, yield and other indicators at seed and fiber mature stage, respectively. The results showed that at fiber mature stage fiber yield was 4183.66-5197.62 kg/ hm2. J8 line had the highest fiber yield (5197.62 kg/hm2) followed by J7 (5150.93 kg/hm2), which displayed superb economic traits. At seed mature stage, seed yield per plant was 2.7-11.9g. J7 line produced most seed (11.9g) and J10 had the second highest yield (11.4g).