To choose Hericium erinaceus strains meet the anniversary produce requirement from 11 different source H. erinaceus strains and provide the cultivation strains for a commercial cultivation facility. Selected biological characteristics, including mycelium growth rate, mycelial antagonistic, the cultivation cycle within a commercial cultivation facility, the shape of fruit body, the length of spine and yield of 11 strains were compared to observe mycelial growth characteristics and the fruiting within a commercial cultivation facility. The results showed that, mycelium growth rate, mycelial antagonistic, the cultivation cycle within a commercial cultivation facility, the shape of fruit body, the length of spine and yield had great difference among 11 strains in the same conditions. 0608 strain exhibited the fastest mycelial growth rate, while 0605 strain exhibited the highest yield and shorter cultivation cycle that conform to the commercial cultivation conditions and Cichang H. erinaceus strain exhibited the minimum deformity rate of fruit body, the best fruit body color and the best shaped mushroom, but the longest cultivation cycle. The requirement for cultivation strain was great different between within a commercial cultivation facility and in the greenhouse. 0605 strain conform to the commercial cultivation conditions. Cichang H. erinaceus strain could be as good quantity spawn for a commercial cultivation facility. Mycelium growth rate of 0605 strain was 0.2865 cm/d on potato dextrose agar (PDA). The cultivation cycle within a commercial cultivation facility was not more than 60 days (two batchs fruit body) and the yield was 217.52 g/bag.