In order to studied the correlation between the growth performance and the activity of GPT, GOT and LDH on Holstein Bull，in order to provide theoretical basis of breeding selection. The experiment was to detect the activities of GPT, GOT and LDH in blood in the period of 6—12 month of twelve healthy Holstein bull calves, comparing the change trend of blood enzyme in each period, and analyzed the correlation between three blood enzyme and their growth performance in corresponding periods, and the regression equation using SPSS software. The results showed that GPT activity had a significant difference in average daily gain of 11 months and 12 months bull, with the coefficient correlation were 0.358, 0.400, respectively; the coefficient correlation of GOT activity and 9 months average daily gain was 0.399 (P＜0.05); the coefficient correlation of LDH activity and 9 months, 11 months average daily gain were 0.550 (P＜0.01), 0.466(P＜0.05), respectively. In conclusion, we could took GPT activity (11 and 12 month periods), GOT activity (9 month period), and LDH activity (9 and 11 month periods) as biochemistry selective markers carrying out the job of selection and breeding.