In this article, elimination of mequindox residue in Cyprinus carpio muscle tissue was studied so as to provide basic data for the quality and security of aquatic products. The experiment was conducted at 15℃. After oral treatment at a single dose of 10 mg/kg, the fish was sampled at certain time and the concentrations of mequindox in muscle were determined by high performance liquid chromatography (HPLC). The results showed that the concentration-time data of mequindox was analyzed with the pharmacokinetics software DAS 2.0. Rosenblueth method of non-compartment model was used to calculate the parameters of mequindox. The main pharmacokinetic parameters were as follows: the terminal elimination half-life (t1/2) was 0.17 h. The times of peak concentration (Tmax) was 0.50 h. The maximum concentration (Cmax) was 761.75 μg/L. The body clearance of the drug was 42.59 (L?h/kg). The mequindox concentration was below detection limits after 1.5 h. The drug concentration versus time curve showed an innormal double-peak phenomenon. The double-peak of concentration meant that there might be hepatoenteral circulation or stomach intestine circulation during the metabolism process. The results showed that the pharmacokinetic characteristics of the mequindox in Cyprinus carpio were as follows: fast absorption, wide distribution, rapid elimination and low drug residues.