To investigate the effects of Forsythoside (FS) on proliferation, NO secretion, TNF-α secretion and phagocytosis of RAW 264.7 cells stimulated by LPS. RAW 264.7 cells in the logarithmic phase were collected and cultured in RPMI1 640 containing 10% fetal blood serum in vitro and the stimulant Lipopolysaccharide (LPS). Different FS concentrations of 40, 80, 160 μg/mL were added individually in culture medium. Cell proliferation was detected by MTT, ELISA and Griess methods were detected TNF-α and NO levels, and cell phagocytosis were analyzed by staining. The results showed that low and medium dose of FS could significantly promoted RAW 264.7 cell proliferation, and the proliferation of cells stimulated by LPS was alleviated at medium and high dose of FS. Compared with LPS group, NO level secreted from LPS-stimulated RAW 264.7 cells treated with FS had not significant effect, but cells secretion of TNF-α was markedly promoted by high dose of FS. FS could enhanced macrophage phagocytosis to chicken red blood cells. FS might had an impact on proliferation, NO and TNF-a secretion, and phagocytic ability of RAW 264.7 cells, which might be one of mechanisms of FS to regulate cellular immune function.