In order to probe the status of the reptile resources and promote wildlife conservation, the reptile resources in Dashiwei karst doline and cave cluster scenic spot in Guangxi were investigated by means of combining methods of line investigation, quadrat investigation and interview investigation from July 2008 to 2011. The results showed that the species diversity of reptile resources in Dashiwei karst doline and cave cluster scenic spot was rich. There were 42 reptiles species belonging to 32 genera, 12 families, 2 orders, accounting for 25.5% of the total 165 reptile species recorded in Guangxi; and that of the 42 species, 38 belong to the Oriental realm and 4 belong to the widespread ones, with no species to the Palaearctic realm. The fauna was mainly composed of species distributed both Central-China and South-China district. The species with quantity dominance are Gekko chinensis,Eumeces chinensis,Sphenomorphus indicus, Takydromus sexlinearus, Xenochrophis piscator, Sinonatrix aequifasciata. According to the results of the surveys,the status of reptile resources was discussed and some protective suggestions are provided.