In order to understand the growth and development of the juvenile of the mud loach and provide basic data for large-scale larval rearing, The morphological development of larval and juvenile mud loach, Missgurnus Arguillicaudatus was examined in a hatchery-reared series. The results showed that when the water temperature ranged between 19.5-22.0℃, the embryonic development took 24-28 hours. The average standard body length of newly hatched larva was 3.04 mm, the body is transparent without any pigments. The mouth and gut haven't formed in the newly hatched larvae. The mouth opened 4 days after hatching and entered a mixed feeding stage. It took 6 days for larva to develop into preflexion larva stage, the yolk is absorbed completely and exdoerophic stage begins. Notochord flexion started on the 10th day. On the 15th day, the hypurals were completed and entered the postflexion larva stage. Transformation from the larval to the juvenile stage occurred on 23th day, the round scales had been formed and the juvenile fish had basic external shape and habits of adult fish. This study is significantly important in conservation and seed breeding of Missgurnus Arguillicaudatus.