The aim was to determine anthracnose in Momordica charantia. The pathogen strain from Hainan Province was identified with morphology and ITS sequence analysis, and the biology characteristics were studied preliminary. The results showed C. gloeosporioides was the pathogens of anthracnose. The alignment analysis indicated that it had the highest homology with the anthracnose pathogen from Taiwan, Shanxi Province and Mexico. The pH values for the mycelial growth was 9-12, with an optimum of pH 10; alternation of light and darkness were conducive to the growth of the mycelial; the spores germinate with pH values 5-8, with an optimum of pH 6; darkness was conducive to the germination of the spores. C. gloeosporioides was the pathogens of anthracnose; the pH values and light condition were the important factors which affected the mycelial growth and spores germination.