To get more the vegetative forms of G.m strains, Sudangrass Hyrid L., as the host plants were inoculated Glomous mosseae (G.m) 12 weeks with six different proportions of culture mediums in pot culture, the influence of the growth parameters of the host plants and breeding parameter of G.m were studied and the influence of physical and chemical properties of mediums and the activity of urease, invertase, acid phosphatase were analyzed. The results showed that the growth parameters of the host plants and breeding parameter of G.m after treatment with medium Ⅵ were higher than others treatments, the content of alkali-hydrolyzed nitrogen (N), available phosphorus (P), available potassium (K), pH, organic matter was 121.5 mg/kg, 13.47 mg/kg, 106.8 mg/kg, 6.9, 32.3% respectively. Correlation analysis showed the colonization rate was positively correlated with infection rate, spore density and which was negatively correlated with alkali-hydrolyzed N, available K, organic matter and was positively correlated with available P, pH of pre-inoculated, meanwhile it was negatively correlated with alkali-hydrolyzed N, available P, available K, organic matter and the activity of urease, invertase acid phosphatase of after-harvest mediums. Infection rate and spore density which were positively correlated with the index of physical and chemical properties, the highest correlation coefficient was available P, then alkali-hydrolyzed N. Infection rate and spore density which was positively correlated with alkali-hydrolyzed N, available P, available K, pH, organic matter, the activity of acid phosphatase, and which were negatively correlated with the activity of urease and invertase of after-harvest mediums. The superior culture substrates for G.m were peat soil, field soil, sand (v/v/v=2:2:1)