The purpose of this research was to establish quality grading standards of Bupleuri Radix seeds in order to control the quality and regulate the seeds’ market supply and demand. In the research, five quality indicators of Bupleuri Radix seeds were tested including germination percentage, 1000-grain weight, neatness, water content and viability using the testing methods identified by our research group; the data were analyzed on the basis of K-means Clustering Algorithm with the SPSS 17.0 statistical analysis software. The research had finally set up the quality grading standards of Bupleuri Radix seeds totally with four indices and three grades, which took germination percentage and the 1000-grain weight as the main criteria, and the neatness and the water content as the reference. Except the seeds with germination percentage below 50%, among 25 samples, there were 8 accessions in the first grade, 11 accessions in the second grade, 6 accessions in the third grade. The standards can provide a reference for Bupleuri Radix seeds quality inspection.