The aim was to investigate the genetic relationship between a new introduced germplasm(Salvia hispanica L.) and other two common members of Salvia L. family ( Salvia splendens Ker-Gawler and Salvia farinacea), and provide the theoretic foundation for distant hybridization and genetic improvement in these three germplasms. In cytogenetics level, karyotypes of Salvia hispanica L., Salvia splendens Ker-Gawler and Salvia farinacea were discussed in this paper. The results showed that chromosome number of normal diploid of Salvia hispanica L. was 2n=12, karyotype formula was 2n=12=2m+2sm+8st, the AS.K% was 74.9%, which belong to “3A” type; chromosome number of normal diploid of Salvia splendens Ker-Gawler. was 2n=44, karyotype formula was 2n=34m+2sm+8st, the AS.K% was 62.4%, which belong to “2A” type; and chromosome number of normal diploid of Salvia farinacea. was 2n=18, karyotype formula was 2n=12m+6sm, AS.K% was 59.0%, which belong to “2A” type. Through the karyotype analysis, there were great differences of chromosomes between Salvia hispanica L., Salvia splendens Ker-Gawler and Salvia farinacea, thus these three members of Salvia L. family had a distant relationship.