For further improving the efficiency of wheat anther culture, and quicken the course of anther culture breeding. One hundred and forty different genotypes of wheat materials and F1, F3 hybrids were used for anther culture, and the Anther Culture Response (ACR) of different genotypes on different hybrid generations were also studied. The results showed that: the callus induction of different genotypes of wheat materials ranged from 2.89% to 41.25%, the average was 3.55%, the frequency of differentiated plantlets ranged from 0% to 70.00%, the average was 33.05%. Among the one hundred and fourteen different genotypes of F1 hybrids, there were one hundred and seven genotypes ,which could induce callus, the frequency of callus induction ranged from 0% to 76.25%, the average were 11.59%, the frequency of differentiated plantlets ranged from 0% to 160.00%, the average was 29.20%. The F3 hybrids all could induce callus, and the frequency of callus induction ranged from 4.58% to 80.83%, the average was 16.39%, the frequency of differentiated plantlets ranged from 0% to 71.43%, the average was 34.99%. So there were significantly difference on callus induction, frequency of differentiated plantlets and green plantlets of different genotypes of wheat materials. According to ACR of different genotypes, selected some materials (varieties) that could be used as bridge parents, such as ‘Ningchun 4’, Jinghong No.2, ‘Jian 63’, Chuanmai 18, Synthderiv.29589, S111, D-27, ‘Jinyan 746-9’, K6-39, and so on.