In order to provide base of large scale propagation and plantation of Mallotus furetianus, factors influenced on in vitro culture and rapid propagation of Mallotus furetianus were studied using stem segments with buds as explants. The results showed that the best sterilization method for the stem segments was dipping in 70% ethanol for 30 s, and then agitating in 0.1% HgCl2 for 8 min. The optimum medium for initiation culture of stem segments was MS with 1.5 mg/L KT and 2.0 mg/L 6-BA. The basic medium 1/2MS with the combination of 6-BA and KT could enhance the multiplication. The suitable multiplication medium was 1/2MS+2.0 mg/L 6-BA +1.5 mg/L KT and the suitable rooting medium was 1/2MS+2.0 mg/L IBA+2.0 mg/L NAA. Using the methods obtained in this experiment, in vitro rapaid propagation of Mallotus oblongifolius can be achieved at the multiplication rate of 3.