Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (14) : 87-93. DOI: 10.11924/j.issn.1000-6850.2011-0541
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Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin

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Abstract

Alcalase was used to hydrolyze plaice skin for preparing collagen peptide, and the superoxide anion radical scavenging activity of this collagen peptide was studied. Effect of the different enzymatic hydrolysis conditions on superoxide anion radical scavenging activity were analyzed by single factor experiment and response surface method. The optimal hydrolytic conditions of plaice skin collagen using Alcalase with high scavenging rate were pH=9.5, enzyme dosage 1581 U/g, concentration of substrate 10 mg/mL, hydrolysis time 6 h,temperature 60℃. The maximum scavenging rate of 75.51% and half scavenging concentration (IC50) of 7.98 mg/mL were obtained.

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response surface method

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Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin. Chinese Agricultural Science Bulletin. 2011, 27(14): 87-93 https://doi.org/10.11924/j.issn.1000-6850.2011-0541

References

王群 郑海涛 葛尧 何计国 .酶法制备鲽鱼鱼皮胶原蛋白肽及其清除超氧阴离子自由基的研究.中国农学通报 ,2011,27(第14期6月): 87-93
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