In order to understand the resouce of Tichoderma species in Shandong Peninsula, one hundred and twenty-seven isolates of Trichoderma spp. were isolated form 59 soil samples that collected in Shandong Peninsula. The morphological characteristics and ITS analysis were used to identify the Trichoderma spp. strains. And the result of ITS analysis was coincident with the morphological characteristics. The result showed that the strains belonged to Trichoderma harzianum, Trichoderma atrotiviride, Trichoderma asperellum, Trichoderma virens, Trichoderma citrinoviride, Trichoderma aureoviride, Trichoderma longibrachiatum. The appearance frequencies of species Trichoderma harzianum, Trichoderma atrotiviride were very high, 26.0% and 25.2% respectively.