We identify soybean cultivar and new strain resistant to Cercospora sojae in the south and north of China to supply good anti-disease resource for breeding for disease resistance and discover new disease-resistant genes. When the soybean goes into State R3 to R4 of its child-bearing period, we evaluate and appraise the resistance of 593 portions of soybean resource proceeding under artificial infection condition. The results showed that the proportion of high resistant material in the north was much higher than that in the south. Moreover, in the highly resistant resources, the proportion of cultivar was obviously higher than that of strain and resource, and the proportion of disease-resistant resources was higher than that of cultivar and strain. The resisted Cercospora sojae was exist in different areas, highly resistant and disease-resistant cultivar was 92 portions and highly resistant and the disease-resistant strain was 57 portions. The results from 200 materials of multi- physiologic seeds showed that there were 55 materials have the ability to resist 5-10 physiologic seeds, and 48 materials to resist 5-7 physiologic seeds. There are materials resistant to Cercospora sojae in the soybean resource from both south and north. The portion of high resistant material in the north is much higher than that in the south. Disease-resistant materials are almost the same between south and north.