In order to search the best effects of the fertilizing treatment of Dendrobium frigdaas ‘Little Bee’, the vegetative growth and flowering have been studied with different time and regime by the orthogonal experiment design L9(33). The results indicated that the vigorous growth period of Dendrobium frigdaas ‘Little Bee’ was from April to August, and the mature period of that was from September to October. Compared with the control, the treatments were more effective for vegetative growth and normal flower induction of Dendrobium frigdaas ‘Little Bee’(P<0.05). Among the treatments, N3P1K3 was the optimal condition to speeding up the growth of the plants, and N1P3K3 was the most effective for in vivo flowering, about two weeks advanced to the emergence for flowers. N at higher concentration was more effective for vegetative growth than that of lower does. The effects of fertilization were in order of N>K>P in the growth period. Meanwhile, N at higher concentration promoted mineral elements of leaf N, P, K absorbing better than that of lower does. However, the perimeter of the shoots and mineral elements of leaf Ca, Mg were lower under N at higher concentration than that of lower dose.