Abstract：This experiment was conducted to investigate the effects of bacillus preparation on product performance and egg quality in layers. Three thousand and six hundred 259-day-old “jing fen yi hao” layers were randomly divided into 2 groups，with 6 replications per group and 300 layers each replication. Layers in control group were fed basal diet. The trail groups were fed diet supplemented with 3% bacillus preparation. The experiment lasted for 40 d. The results showed that there were no significant effects of trail group on product performance and egg quality of layers in this experiment. Egg quality was improved by trail group.