The seed germination rate of the rare medical plant Nauclea officinalis under natural conditions is extremely low, and it is limiting factor to artificial propagation and resource conservation. Effects of Atonik and GA treatments on seed germination of Nauclea officinalis were determined and the treatments of both growth regulators obviously promoted seed germination of Nauclea officinalis. The optimum concentrations and time of the treatments were the 3000-4000 times solution Atonik soaking for 10-12 h, 400 mg/L GA soaking for 12 h. Compared with the control, the germination time were 9 and 8.6 day earlier, the germination potentials were increased to 28.3%-30.3% and 32%, and the germination rate were 32.3%-30.3% and 34%, respectively.