The probit analysis which had used for half a century, has been taken place by TDM model investigation in bioactivity assay as a neglect of dose and time dependent manner. The TDM model and growth curve of S-type model were introduced to analyze contact toxicity of squalene, β-sitosterol and scopoletin, which were extracted from the roots of Stellera chamaejasme, against Tetranychus cinnabarinus. The results showed that the order of sensitive degree to concentration of different active substrates was scopoletin>β-sitosterol>squalene. Each acaricidal substances reached rapidly mortality periods were that β-sitosterol 12 h, squalene 6 h and scopoletin 2 h. The theoretical periods of rapidly mortality were equal to observed value. The LC50 values of different substances analyzed by TDM model was squalene 13.101 mg/mL, 9.288 mg/mL, and scopoletin 1.318 mg/mL, which were 1.321, 1.110, 1.040 times as LC50 value calculated by probit analysis, respectively. The LT50 value of scopoletin (2.237 h) was the minimum, and squalene (7.395 h) was the maximum in this study. Both Gompertz and Logistic model had fitting effects to the changes of mortality in the maximum concentrations of experiment design, and the Logistic model had the best fitness. The preliminary conclusions were that all of acaricidal substances in this research had significant control effects, and the onset time of scopoletin treatment was the earliest and the theoretical effective period against Tetranychus cinnabarinus was the shortest, while the onset time of β-sitosterol were the latest and the effective period was the longest.