In order to inquire the meaning and feasibling on transfer the vineless character from C. moschata into C. maxima and C. pepo, the interspecific crossing between C. moschata materials with vineless character and C. maxima materials or C. pepo materials was conducted, we studied the interspecific genetic development on vineless character by the RAPD molecular marker and field investigation. Genetic performance of the vineless character of Cucurbita moschata in three mainly cultivated species in Cucurbita was studied in this paper. The results showed that the segregation ratios of the vineless character in F1 group, F2 group and BC1F1 group of the interspecific hybrids fit the Mendelian law. It was different from intraspecific hybridization that the first filial generation between vineless C. moschata and the other vine pumpkin species showed that the internode elongated and the inheritance belonged to incomplete dominant inheritance. The unique vineless character of C. moschata was transferred to C. pepo and C. maxima by the means of interspecific hybridization and the brand-new germplasm resources were created.