For the purpose of the selection the superior flue-cured tobacco cultivars for Lijiang area, the field properties and dissecting structure of leaves of ten newly introduced flue-cured tobacco cultivars (‘Yunyan98’, ‘Yunyan110’, ‘Yunyan113’, ‘Yunyan114’, ‘Yunyan115’, ‘Yunyan202’, ‘H3’, ‘MSK326’, ‘T29’ and ‘XYN87’) were measured. The results showed: (1) The new ten flue-cured tobacco cultivars can adapt the local ecological environment and farming system well, ‘T29’ had the longest growth period, ‘T29’, ‘XYN87’ and ‘H3’ had the best agronomic characters. (2) ‘Yunyan110’, ‘XYN87’, ‘MSK326’ and ‘H3’ had the best leaves’ dissecting structure, however, ‘Yunyan113’ and ‘T29’ were on the contrary; (3) Coefficient of variation of palisade/spongy tissue thickness ratio was the biggest, which was 26.51%, but leaf thickness and palisade/leaf thickness ratio were the least, that were 15.93% and 13.42%, respectively. Palisade tissue thickness had significant positive correlation with leaf thickness, palisade/spongy tissue thickness ratio and palisade/leaf thickness ratio, had positive correlations with spongy tissue thickness. The study also indicated improving cultural practice can make the dissecting structure of flue-cured tobacco leaves that had good agronomic characters better.