In the recent years, the molecular techniques developed rapidly, and it was used in Sorghum diversity. DNA mo1ecular markers had many advantages compared with the traditional methods, so they were efficient techniques in Sorghum research. This paper briefly introduced six major types of DNA molecular markers, and summarized the present situations of molecular markers in relevant study and application in sorghum breeding in past years, such as the construction of genetic spectrum and gene locating, association of genetics, mutants. Finally, the perspective and the question of molecular marker’s application in Sorghum genetic breeding in the future were discussed.