In order to find the leaf quality characteristics of major grown tobacco varieties for the purpose of producing special quality leaves, we selected four tobacco varieties, ‘Yunyan 85’, ‘K326’, ‘Guiyan 201’ and ‘Nanjiang 3’, which were widely grown in Guizhou, to study the characteristic of leaf cuticular components. The major cuticular components from cured leaves at three plant positions of four flue-cured tobacco varieties grown in Guizhou were analyzed after extracted with methylene chloride. The results showed that the major cuticular components of cured leaf were nicotine, neophytadiene, trichome exudates and alkanes, the amount of which varied among various varieties. The order of total cuticular components, neophytadiene and trichome exudates for four varieties, from low to high was ‘Nanjiang 3’,‘K326’,‘Guiyan 201’and‘Yunyan 85’. The average content of rentinol was roughly equal to the total amount of all other terpenes, the average content of cembratriene-diol was more than that of cembratriene-ol, the level of α-cembratriene-diol was more than twice as that of β-cembratriene-diol. The average levels of alkanes from middle position leaves of all varieties were the lowest; further research is needed to explore the causes.