Abstract: The effect of Fusarium oxysporum f.sp. cubense on the defensive enzymes of different banana variety was researched in this paper. The results showed that the varieties of SOD activity showed the negative correlation of resistance of banana to Fusarium oxysporum f.sp. cubense. There was no interrelationship between the resistance of banana and the variety trend of PO and endo-chitinase activity. The variety trend of PAL, β-1,3-glucanse and exo-chitinase displayed the resistance of banana to the pathogen, and they could be use as important biochemical parameters of banana to resist the Fusarium oxysporum f.sp. cubense.