Abstract: In order to obtain the optimum condition of the Rehmannia glutinosa Libosch polysaccharide microwave-assisted extraction techniques, the content of polysaccharide in Rehmannia glutinosa Libosch was measured by phenol-H2SO4 colorimetric method. This paper studied the effect of treatment time of microwave, power of microwave and rate of water to material through devised different factors. The optimum microwave extraction condition was determined by response surface analysis. The results showed the treatment time of microwave, power of microwave and the rate of material to material had a remarkable effect on obtain of polysaccharide in Rehmannia glutinosa Libosch. When the power of microwave was 720W, the treatment time of microwave was 210s, the rate of material to water was 6:1, the extraction rate of polysaccharide in Rehmannia glutinosa Libosch was 61.9%. This result also had a good reproducibility.