Leaf of three varieties of different leaf-type of African violet（Saintpaulia ionantha）was used as explants to establish a rapid and effective tissue culture regeneration system. The results showed the best culture media of leaf callus induction were MS+6-BA 0.5 mg/L+NAA 0.3 mg/L and MS+6-BA 0.5 mg/L+NAA 0.5mg/L. After 4 weeks cultivation, callus induction rate reached to 100%, and buds occurred on almost every callus. After subculture on the medium MS+6-BA 0.5 mg/L+NAA 0.3 mg/L, about 30 buds could be observed on one callus. There were no obvious differences in callus and bud induction of in different cultivars. The proper culture medium for rooting culture was 1/2MS+0.2mg?L-1 NAA for varieties A and C, but 1/2MS+0.5mg?L－1IBA for B varieties. vermiculite was the optimum medium for test-tube plantlet after transplant, and 1/4 MS(large elements and trace element) should be used as foliar fertilizer, and the survive rate of the root regeneration plant was over 90%. After transferred to humus, above 95% of plantlet could survive. After 3 month of cultivation, the African violet burst into blossom. The results above provided the basic data for industrialized production of test-tube plantlet of African violet.