Abstract: Objective: The purpose of this study was to grope for antibacturial ability and virulence intensity of Honggumycin.Methods: Honggumycin was used as experimental material and nine kinds of bacterium was used as the tested strains separately. The antibacterium and virulence intensity of Honggumycin to bacterium is tested by the method of turbidity comparing. Results: In all the nine tested bacterium,Honggumycin has antibacterium for the eight bacterium except for Aeromonas hydropila. EC50 and EC90 of the eight bacterium were as follows: Staphylococcus aureus, 0.002mg/L and 0.017mg/L, Bacillus cereus, 0.02 mg/L and 0.19 mg/L, Bacillus megaterium, 0.08 mg/L and 0.44mg/L, Sh.flexneri, 0.17 mg/L and 1.87 mg/L, Pseudomonas solanacearum, 0.26 mg/L and 2.3 mg/L, Escherichia coli,0.73 mg/L and 4.49 mg/L, Xanthomonas citri, 0.73 mg/L and 5.0 mg/L, Xanthomonas oryzae, 124 mg/L and 226mg/L.Conclusion: Honggumycin have a very strong inhibition to eight of the rest kinds of the tested bacteria respectively.