Based on the standard acid polyacrylamide gel electrophoresis of wheat gliadin was published by ISTA in 1986, combined with the actual circumstance of national laboratories, and explore the improved methods of the acid polyacrylamide gel electrophoresis of wheat gliadin of the reagents and equipment for our country. This method overcame the many restrictions of the standard method, such as the poor gel toughness, the gel desquamated uncomfortably, low resolution, troublesome identification of the bands, less efficiency of the experiment.,and suited the rapid identification and the consanguinity analysis of the purity of the Chinese wheat breed and so on.