Abstract：To decrease the reliance on peat of flue—cured tobacco seedling,the new type medium which substituted completely the peat in the substrate formula was studied.The results showed that the seedling stages of 2 new mediums were the same of the check,and the growth and development of seedling of T1 was the worst,the difference of T2 and check were not obvious.The seedling ratio of the check was ranked the first ,of T2 was the second , of T1 was the last.The matured seedling diathesis quality of T2 and check, with more lateral roots,was better than that of T1.The yield, produce value,average price super class leaf ratio,high and middle grade leaf ratio of T2 were the highest,but contrasted with the check,the difference was not significant.