The germinating potential, germination rate, Coleoptile length, radicle length and the activities of SOD and POD, relative water content and MDA content in 10 hexaploid triticale varieties were analyzed under 20%PEG-6000(W/V) stress, then drought-resistance coefficient of each trait was calculated. After clustered according to drought-resistance coefficient , 10 varieties were divided into two main groups: one group is S1, S3, S5, S8, S10, which has strong draught-resistant; The other group is S2, S4, S6, S7, S9, which has weak draught-resistant. On the other hand, 10 varieties were divided into three groups: S4, S5; S1, S2, S3, S6, S7, S8, S9; S10; using RAPD markers, based on 0.34 threshold value. In comparison with two clustering results, the strong draught-resistant varieties were scattered in three groups clustered on RAPD markers, indicating that these molecular markers are not related to draught resistance of the varieties. However, the results also showed that these drought-resistant varieties have quite different genetic diversity, which can provide a theoretical basis for using these variety resources in wheat or triticale’s draught-resistant breeding.