Experiment was conducted with the dormancy buds of Taishanganying, the effect of plant growth regulators on the initial culture、multiplication and root regeneration were analyzed and studied. The results showed that on the medium of MS+6-BA1.0mg/L+IBA0.1 mg/L the best of clump shoot was inducted; MS medium with 0.5mg/L 6-BA and 0.1mg/L ZT was the most suitable for the propagation of Taishanganying and the propagation rate reached 6.8times.The effective number of new shoot was up to 80.2%.On the other hand,the good medium to induce root was 1/2 MS+IBA 0.5mg/Lwith 80% of rooting rate and 4.54cm of average root length. test-tube saplings having taken root were ransplanted in soil texture hased on river sand and turf.Small arch plastic shed in conservatory kept moisture and temperature ,the survival rate of transplant came up to more than 70%.