The middle-microelements nutrients in 557 soil samples collected from 3 regions of tobacco-growing areas in Fujian provinces were analyzed．The results showed that the average content of exchangeable Ca, exchangeable Mg, available sulfur, water-soluble B , water-soluble Cl , available Zn, Cu, Fe were 1059.3±395.3mg/kg、171.2±125.3 mg/kg、34.1±21.7 mg/kg、0.18±0.14mg/kg、23.5±11.8 mg/kg、1.99±1.30mg/kg、2.84±1.56mg/kg , 216.6±98.2mg/kg , respectively。26.2% of soil in tobacco-growing areas were short of Ca , 12.4% of soil in tobacco-growing areas were short of Mg, and Calcium magnesium phosphate fertilizer or MgO should be applied. 42.2% of soil in tobacco-growing areas were short of S, the water-soluble B of soil in tobacco-growing areas was deficiency in general, the water-soluble Cl of soil in tobacco-growing areas was suitable for tobacco growth, available Cu, Fe, Zn of soil in tobacco-growing areas was enrichment . The fertilization suggestion of spraying B was recommended.