AFLP Analysis of 7 SH Line Apple Rootstocks

Pu Nana, Du Guoqiang, Li Mingyuan, Zhang Jianwen, Ma Baokun

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (6) : 141-141. DOI: 10.11924/j.issn.1000-6850.0706141
生物技术科学

AFLP Analysis of 7 SH Line Apple Rootstocks

  • Pu Nana, Du Guoqiang, Li Mingyuan, Zhang Jianwen, Ma Baokun
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Abstract

The improved method of CTAB-DNA isolation was used to extract genomic DNA from leaves of 7 SH lines (Malus domestica Borkh. X Malus. Honanensis Rehder), which are used as apple rootstocks. The materials were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol in order to detect blood relationship and genetic diversity. 20 pairs primers were selected from 143 primer pairs(3+3)to amplify the genetic DNA. Total 458 distinct amplification bands were obtained, 261 of them were polymorphic markers and the polymorphic frequency was 57%. UPGMA cluster analysis based on simple matching coefficient (SM) divided the 7 SH line apple rootstocks into 2 groups at 0.58 level.

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Apple;Rootstock;AFLP;Genetic diversity;Cluster analysis

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Pu Nana, Du Guoqiang, Li Mingyuan, Zhang Jianwen, Ma Baokun. AFLP Analysis of 7 SH Line Apple Rootstocks. Chinese Agricultural Science Bulletin. 2007, 23(6): 141-141 https://doi.org/10.11924/j.issn.1000-6850.0706141

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