The polymorphisms of two sites of GH gene in the 5′ flanking region from 168 Huanghuai goats、Changjiangsanjiaozhou white goats and Boer goats were analyzed by PCR-SSCP, At the same time, the three communities were analyzed to check whether they were in balance. Results: both of the two sites of GH gene were polymorphic, there were six genotypes and two mutations with the first pair primers, three genotypes and three mutations with the second pair primers. communities analysis shows that all the three breeds in the first site were in imbalance, which suggests that artificial selection might be possible in this site; and all the three breeds in the second site were in balance. Furthermore, GH gene in the 5′ flanking sequence were analyzed by bioinformatics software assay, which shows that transcription factor combining sites with high score, such as IA-1、PIT-1、Gsh-2、C/EBP et al, exist in the polymorphic sites, and the mutation of T→C(264) results in the disappearance of PIT-1 and Gsh-2 combining sites, Further studies need to be carried out to check such sites will affect the expression of GH gene, and the growth performance of goat.
Key words
PCR-SSCP;Goat;GH gene;Polymorphism;Bioinformatics
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References
[1]Elio F De Palo, Vincenzo De Filippis, Rosalba Gatti et al. Growth hormone isoforms and segments/fragments:Molecular structure and laboratory measurement.Clinica Chimica Acta, 2006,364:67-76.
[2]Chung E R, Lee C S. A previously unreported DraI polymorphism within the regulatory region of the bovine growth hormone gene and its association with growth traits in Korean Hanwoo cattle. Animal Genetics Volume, 2004,35(2):152-158.
[3]王文君,陈克飞,任军,等.中外不同猪品种生长激素基因遗传多态性检测[J].农业生物技术学报,2003,11(1):103-104.
[4]聂庆华,张细权,杨关福,等.鸡生长激素基因的PCR-RFLP分析[J].农业生物技术学报,2001,9(3):282-285.
[5]MIN Ling-Jiang, LI Mei-Yu, SUN Guo-Qiang, et al. Relationship Between Polymorphism of Growth Hormone Gene and Production Traits in Goats. Acta Genetica Sinica, 2005,32(6):650-654.
[6]Marques P X, Pereira M, Marques M.R, et al. Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research, 2003,50:177-185.
[7]Malveiro E, Pereira M, Marques P.X, et al. Polymorphisms at the five exons of the growth gene in the algarvia goat: possible Association with milk traits[J].Small Ruminant Research, 2001,41:163-170.
[8]李美玉,闵令江,孙国强,等.山羊生长激素基因5′调控区的多态性分析[J].遗传,2004,26(6):831-835.
[9]Mathias SGierl, Nikolaos Karoulias, Hagen Wende, et al. The Zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic βcells and intestinal endocrine cells. GENES & DEVELOPMENT, 2006,20:2465-2478.
[10]Cohen L E, Wondisford F E, Radovick S. Role of Pit-1 in the gene expression of growth hormone, prolactin, and thyrotropin[J]. Endocrinol Metab Clin North Am, 1996,25(3):523-540.
[11]Zhao Q, Davis M E, Hines H C. Associations of polymorphisms in the Pit-1 gene with growth and carcass traits in Angus beef cattle. Animal Science, 2004,82:2229-2233.
[12]Sonja Kriks, Guillermo M Lanuza, Rumiko Mizuguchi, et al. Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord[J]. Development, 2005,132(13):2991-3002.
[13]Jacob K K, Stanley F M. CCAAT/enhancer-binding protein alpha is a physiological regulator of prolactin gene expression. Endocrinology, 1999,140(10):4542-4550.
[14]Enwright J F III, Kawecki-Crook M A, Voss T C, et al. A PIT-1 homeodomain mutant blocks the intranuclear recruitment of the CCAAT/ enhancer binding protein alpha required for prolactin gene transcription. Mol Endocrinol, 2003,17(2):209-222.
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Footnotes
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