In order to reveal the correlation among the fertilizer rate, topping stage and main chemical component influencing of tobacco in different ecology areas, there carried out the tests of fertilizer amount, which was 72kg/hm2, 90kg/hm2, 108kg/hm2 respectively, and the tests of topping at buttoning stage, topping, at the early bloom and topping at full blooming stage. The results indicated there had significantly difference among content of total sugar, total nitrogen, protein and potassium in different ecology spots. A significantly difference was observed among total sugar, reducing sugar, total nitrogen and protein at the best fertilizer amount level and less than 20% best fertilizer amount treatment. There had no significantly difference among total sugar, reducing sugar, total nitrogen and protein by the three topping treatments. There obtained a best significant difference among total sugar and reducing sugar at different stalk position, which was lower leaf>middle leaf>upper leaf. A best significant difference was observed in potassium content at different stalk position, which was lower leaf>middle leaf>upper leaf. A rare affection on Potassium uptake by fertilizer and topping and a high genetical stability were obtained.