用黔糖4号作供试材料,选择蔗茎嫩梢部茎腋芽作接种外植体进行离体培养;外值体消毒采用75%酒精浸泡8~10S后用0.1%HgCl2消毒15min复合消毒,多次清洗再接种;腋芽诱导培养基采用(液态)1/2MS+0.25mg/L(6-BA);丛芽诱导增殖培养基采用(固态)MS+2.5mg/L(L-BA)+0.2mg/L(KT
黔糖号;甘蔗腋芽;离体快繁;种苗
用黔糖4号作供试材料,选择蔗茎嫩梢部茎腋芽作接种外植体进行离体培养;外值体消毒采用75%酒精浸泡8~10S后用0.1%HgCl2消毒15min复合消毒,多次清洗再接种;腋芽诱导培养基采用(液态)1/2MS+0.25mg/L(6-BA);丛芽诱导增殖培养基采用(固态)MS+2.5mg/L(L-BA)+0.2mg/L(KT
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