利用ISSR技术对香蕉30个品种的遗传多态性进行了分析。从60个随机引物中筛选出8个多态性引物，共扩增出54条DNA带，其中44条为多态性带，占81.5%，平均每个引物扩增的DNA带数为6.75条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵，用MEGA2.0软件对香蕉

香蕉；品种；ISSR；遗传多样性

Chinese Agricultural Science Bulletin ›› 2006, Vol. 22 ›› Issue (3) : 366-366. DOI: 10.11924/j.issn.1000-6850.0603366
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利用ISSR技术对香蕉30个品种的遗传多态性进行了分析。从60个随机引物中筛选出8个多态性引物，共扩增出54条DNA带，其中44条为多态性带，占81.5%，平均每个引物扩增的DNA带数为6.75条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵，用MEGA2.0软件对香蕉

  • 香蕉；品种；ISSR；遗传多样性
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Zhang Rulian, Fu Xiaoxia, Hong Caixiang, Qi Zhiping, Xie Zhenyu, Xie Zisi

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(Tropical crops genetic resources institute;CATAS;Danzhou Hainan 571737)

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香蕉；品种；ISSR；遗传多样性. 利用ISSR技术对香蕉30个品种的遗传多态性进行了分析。从60个随机引物中筛选出8个多态性引物，共扩增出54条DNA带，其中44条为多态性带，占81.5%，平均每个引物扩增的DNA带数为6.75条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵，用MEGA2.0软件对香蕉. Chinese Agricultural Science Bulletin. 2006, 22(3): 366-366 https://doi.org/10.11924/j.issn.1000-6850.0603366

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ISSR assessment of genetic variations in 30 varieties of banana(Musa spp.)was carried out .Eight primers were screened from 60 arbitrary primers, and a total of 54 DNA bands were amplified，among which 44(81.5%)were polymorphic. The average number of DNA bands amplified by each primer was 6.75，and the average genetic distance among 30 varieties was 0.313. Based on UPGMA cluster analysis of 54 DNA bands amplified by 8 primers a DNA molecular dendrogram was established，which divided the 30 varieties into four groups: group A, B, C and D.
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