This dissertation used 6 celery lines. in 14℃/10℃(d/n) and 10℃/10℃(d/n), the low temperature stress tolerance of lines 10, 14, 16, 20, 22, 24 are evaluated by mensurate weight growing, stability of cell membrane, dissociative praline, dissolvable sugar, dissolvable protein. The main results are as follows: It is good putting celery plants in 10℃/10℃(d/n) when we study the changes the celery materials make under low temperature stress.At the same time, when we studied the cold resistance, 10℃/10℃ will be a good condition to deal with the plants. Get one material (24) having good tolerance to low temperature. From monomial mensurated results, we can see that the sequences of the low temperature stress tolerance of several lines are different, but the low temperature stress tolerance of some lines is steady, such as 24, 14 and etc, So we can account for the trend of the low temperature stress tolerance of lines.