In 2002and 2003,as result of climate, cotton seedling disease was more serious in the cotton districts of Henan Province. By studying on function of temperature and seed coating formulations, to seed cotton to find method of tackling the problem of cotton seedling emergence. On the condition of low temperature and normal temperature treating the seed cotton and the delinted seed cotton by H2SO4 with Carboxin. Thiram, Amicarthi Thram, Carbendazim, Fludioxonil. By surveying seedling emergence rate, seedling disease rate, seedling death rate and seeding stage,to find the superior treatment. The results demonstrated that treated seed cotton by Carboxin.Thiram was the best in these seed coatings.